JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729812.1
Database:	nt	Go to original record
Description:	Tamarix meyeri tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tamarix meyeri
Original size:	1041 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	595	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaacccc
101   ggaattaaaa aaaaagggca atcctgagcc cactcctgtt ttcccaaaag
151   ttaaaaaaaa aaaaaattat taaagcgaga ataaataatg gataggtgca
201   gagactcaac ggaagctgtt ctaacaaatg gagtttactg tattattata
251   agaattcttc cataaaaact gcaaaaaaaa gatgaagaag aaccctatat
301   ctagataaat acgtactaaa atattctcaa aaataaaaaa acgtattaat
351   gacgggcaaa tatgtctttt ttaccttttg tctaggaaaa aatgtaagaa
401   tattaaatta tcaaagtatt cactccatgg tctgatagat cctttttttt
451   gtacgaactg ctcaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaatactg acaacaatga aatttatagt atgaggaaaa tccgtcgact
551   ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details