JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729811.1
Database:	nt	Go to original record
Description:	Tamarix karakalensis tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tamarix karakalensis
Original size:	1036 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	588	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaagta
151   aaaaaaaaat tattaaagcg agaataaata atggataggt gcagagactc
201   aacggaagct gttctaacaa atggagttta ctgtattatt ataagaattc
251   ttccataaaa actgcaaaaa aaagatgaaa aaaaacccta tatctagata
301   aatacgtact aaaatattct caaaaataaa aaaacgtatt aatgacgggc
351   aaatatgtct tttttacctt ttgtctagga aaaaatgtaa taatattaaa
401   ttatcaaagt attcactcca tggtctgata gatccttttt tttgtacaaa
451   ctgctcaatc ggacgaaaat aaagatagag tcccattcta catgtcaata
501   ctgacaacaa tgaaatttat agtatgagga aaatccgtcg actttagaaa
551   tcgggagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details