JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729807.1
Database:	nt	Go to original record
Description:	Myricaria bracteata tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Myricaria bracteata
Original size:	1056 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	602	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatggggca atcctgagcc aactcctgtt ttcaaaaatc
151   aaaaagtaaa aaaaaattct taaagcgata ataaataatg gataggtgca
201   gagactcaac ggaagctgtt ctaacaaatg gagtttactg tgttgttata
251   agaattattc cataacaact gcaaaaaaaa gatgaagaag aaccctatat
301   ctagataaat acgtactaga aatactttaa aaaataaaaa aacgtattaa
351   tgacggccaa aatatgtctt ttttaccctt ttgtatagga aaaaatggaa
401   gaatattaat ttataaaagt attcactcca tggtctgata gatctttttt
451   ttttttttga cgaactgctc aatcggacga gaatgaagat agagtcccat
501   tctacatgtc aatactgaca acaatgaaat ttatagtatg aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details