JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729805.1
Database:	nt	Go to original record
Description:	Reaumuria vermiculata clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria vermiculata
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	578	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   aaaaaaaatt attaaagtga gaagaaatga tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgcgttgtta taagaattct
251   tccatcaaaa cttaaaaaaa gatcaagaca aaccctatat ttagataaat
301   acgtactgaa atactctaaa aaataaaaaa cgtcttaata ataatggcgg
351   ccaaaatatg tcttttttat atgaaaaatg gaagaatatt catttatcaa
401   agtattcgct ccatggtctg atagattttt ttttgacgaa ctgctcaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ctgacaacaa
501   tgaaatttat agtatgagga aaatccgtcg actttagaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details