JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729803.1
Database:	nt	Go to original record
Description:	Reaumuria turkestanica clone 7 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria turkestanica
Original size:	1029 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	584	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaaaa ttattaaagt gagaagaaat gatggatagg tgcagagact
201   caacggaagc tgttctaaca aatggagttg actgcgttgt tataagaatt
251   cttccatcaa aacttaaaaa aagatcaaga caaaatttag aatttagatt
301   tagataaata cgtactgaaa tactctaaaa aagaaaaaaa cgtcttaatg
351   ggcggacaaa atatgtcttt ttatatgaaa aagtggaaga atattcattt
401   ataaaagtat tcgctccatg gtccgataga tctttttttt gacgaactgc
451   tcaatcggac gagaatgaaa atagagtccc attctacatg tcaatactga
501   caacaatgaa atttatagta tgaggaaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details