JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729799.1
Database:	nt	Go to original record
Description:	Reaumuria turkestanica clone 3 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria turkestanica
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	582	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaagat
151   aaaaaaaaat tattaaagtg agaagaaatg atggataggt gcagagactc
201   aacggaagct gttctaacaa atggagttga ctgcgttgtt ataagaattc
251   ttccatcaaa acttaaaaaa agatcaagac aaaccctata tttagattta
301   gataaatacg tactgaaata ctctaaaaaa gaaaaaaacg tcttaatggg
351   cggacaaaat atgtcttttt atatgaaaaa gtggaagaat attcatttat
401   aaaagtattc gctccatggt ccgatagatc ttttttttga cgaactgctc
451   aatcggacga gaatgaagat agagtcccat tctacatgtc aatactgaca
501   acaatgaaat ttatagtatg aggaaaatcc gtcgacttta gaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details