JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729790.1
Database:	nt	Go to original record
Description:	Reaumuria sogdiana clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria sogdiana
Original size:	1036 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	591	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaaaa attattaaag tgagaagaaa tgatggatag gtgcagagac
201   tcaacggaag ctgttctaac aaatggagtt gactgcgttg ttataagaat
251   tcttccatca aaacttaaaa aaagatcaag acaaacccta tatttagaat
301   ttagatttag ataaatacgt actgaaatac tctaaaaaag aaaaaaacgt
351   cttaatgggc ggacaaaata tgtcttttta tatgaaaaag tggaagaata
401   ttcatttata aaagtattcg ctccatggtc cgatagatct tttttttgac
451   gaactgctca atcggacgag aatgaagata gagtcccatt ctacatgtca
501   atactgacaa caatgaaatt tatagtatga ggaaaatccg tcgactttag
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details