JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729788.1
Database:	nt	Go to original record
Description:	Reaumuria reflexa clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria reflexa
Original size:	1047 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	590	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   caaaaaaaaa ttattaaagt gagaagaaat gatggatagg tgcagagact
201   caacggaagc tgttctaaca aatggagttg actgcgttgt tataagaatt
251   cttccatcaa aacttaaaaa aagatcaaga caaaccctat atttagaatt
301   tagatttaga taaatacgta ctgaaatact ctaaaaaaga aaaaaacgtc
351   ttaatgggcg gacaaaatat gtctttttat atgaaaaagt ggaagaatat
401   tcatttataa aagtattcgc tccatggtcc gatagatctt ttttttgacg
451   aactgctcaa tcggacgaga atgaagatag agtcccattc tacatgtcaa
501   tactgacaac aatgaaattt atagtatgag gaaaatccgt cgactttaga
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details