JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729784.1
Database:	nt	Go to original record
Description:	Reaumuria persica clone 1 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria persica
Original size:	1025 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	582	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   aaaacaaatt cttaaagtga gaagaaataa tggataggtg cagagactca
201   acggaagttg ttctaacaaa tggagttgac tgtgttgtta taagaattct
251   tccatcaaaa cttaaaaaaa gatcaataaa aaccctatat ttagataaat
301   acgtactgaa atactctaaa aaagaaaaaa acgtcttaat gataatgacg
351   gccaaaatat gtctttttta tatgaaaaaa tggaagaata ttcatttatc
401   aaagtattcg ctccatggtc tgatagatct ttttttttga cgaactgctc
451   aatcggacga gaatgaagat agagtcccat tctacatgtc aatactgaca
501   acaatgaaat ttatagtatg aggaaaatcc gtcgacttta gaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details