JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729782.1
Database:	nt	Go to original record
Description:	Reaumuria korovinii tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria korovinii
Original size:	1018 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	585	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaaaa ttcttaaagt gagaagaaat gatggatagg tgcagagact
201   caacggaagc tgttctaaca aatggagttg actgcgttgt tataagaatt
251   cttccatcaa aacttcaaaa aagatcaaga caaacattta gaatttagat
301   ttagataaat acgtactgaa atactctaaa aaagaaaaaa acgtcttaat
351   gggcggacaa aatatgtctt tttatatgaa aaagtggaag aatattcatt
401   tataaaagta ttcgctccat ggtccgatag atcttttttt tgacgaactg
451   ctcaatcgga cgagaatgaa gatagagtcc cattctacat gtcaatactg
501   acaacaatga aatttatagt atgaggaaaa tccgtcgact ttagaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details