JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729781.1
Database:	nt	Go to original record
Description:	Reaumuria kaschgarica tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria kaschgarica
Original size:	1016 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	575	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   caaacaaatt cttaaagtga gaagaaataa tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgtgttgtta taagaattct
251   tccatcaaaa cttaaaaaaa gatcaagaaa accctatatt tagataaata
301   cgtactgaaa tactctaaaa aagaaaaaaa cgtcttaatg acggccaaaa
351   tatgtctttt ttatatgaaa aaatggaaga atattcattt atcaaagtat
401   tcgctccatg gtctgataga tctttttttt tgacgaactg ctcaatcgga
451   cgagaatgaa gatagagtcc cattctacat gtcaatactg acaacaatga
501   aatttatagt atgaggaaaa tccgtcgact ttagaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details