JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729779.1
Database:	nt	Go to original record
Description:	Reaumuria hypericoides clone 1 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria hypericoides
Original size:	1013 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	576	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   caaaaaaaat tattaaagtg agaagaaatg atggataggt gcagagactc
201   aacggaagct gttctaacaa atggagttga ctgcgttgtt ataagaattc
251   ttccatcaaa acttaaaaaa agatcaagac aaaccctata tttagagaaa
301   tacgtactga aatactctaa aaaaggaaaa aaacgtctta atggcggcca
351   aaatatgtct ttttatatga aaaagtggaa gaatattcat ttataaaagt
401   attcgctcca tggtccgata gatctttttt ttgacgaact gctcaatcgg
451   acgagaatga agatagagtc ccattctaca tgtcaatact gacaacaatg
501   aaatttatag tatgaggaaa atccgtcgac tttagaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details