JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729778.1
Database:	nt	Go to original record
Description:	Reaumuria hirtella clone 3 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria hirtella
Original size:	1031 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	581	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaatt
151   aaaaaaaaat tcttaaagtg agaagaaatg atggataggt gcagagactc
201   aacggaagct gttctaacaa atggagttga ctgcgttgtt ataagaattc
251   ttccatcaaa acttaaaaaa agatcaagac aaaccctata tttagataaa
301   tacgtactga aatactctaa aaaataaaaa acgtcttaat aataatggcg
351   gccaaaatat gtctttttat atgaaaaagc ggaagaatat taatttataa
401   aagtattcgc tccatggtct gatagatctt ttttttttac gaactgctca
451   atcggacgag aatgaagata gagtcccatt ctacatgtca atactgacaa
501   caatgaaatt tatagtatga ggaaaatccg tcgactttag aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details