JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729777.1
Database:	nt	Go to original record
Description:	Reaumuria hirtella clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria hirtella
Original size:	1034 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	584	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaatt
151   aaaaaaaaaa attattaaag tgagaagaaa tgatggatag gtgcagagac
201   tcaacggaag ctgttctaac aaatggagtt gactgcgttg ttataagaat
251   tcttccatca aaacttaaaa aaagatcaag acaaacccta tatttagata
301   aatacgtact gaaatactct aaaaaataaa aaacgtctta ataataatgg
351   cggccaaaat atgtcttttt atatgaaaaa gcggaagaat attaatttat
401   aaaagtattc gctccatggt ctgatagatc tttttttttt tacgaactgc
451   tcaatcggac gagaatgaag atagagtccc attctacatg tcaatactga
501   caacaatgaa atttatagta tgaggaaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details