JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729776.1
Database:	nt	Go to original record
Description:	Reaumuria hirtella clone 1 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria hirtella
Original size:	1046 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	579	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaatt
151   aaaaaaaatt attaaagtga gaagaaatga tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgcgttgtta taagaattct
251   tccatcaaaa cttaaaaaaa gatcaagaca aaccctatat ttagataaat
301   acgtactgaa atactctaaa aaataaaaaa cgtcttaata ataatggcgg
351   ccaaaatatg tctttttata tgaaaaagcg gaagaatatt aatttataaa
401   agtattcgct ccatggtctg atagatcttt ttttttacga actgctcaat
451   cggacgagaa tgaagataga gtcccattct acatgtcaat actgacaaca
501   atgaaattta tagtatgagg aaaatccgtc gactttagaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details