JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729775.1
Database:	nt	Go to original record
Description:	Reaumuria fruticosa clone 2 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria fruticosa
Original size:	1034 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	592	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag agaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaaaagt
151   caaacaaatt cttaaagtga gaagaaataa tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgtgttgtta taagaattct
251   tccatgaatt cttccatcaa aacttaaaaa aagatcaaga aaaaccctat
301   atttagataa atacgtactg aaatactcta aaaaagaaaa aaacgtctta
351   atgataatga cggccaaaat atgtcttttt tatatgaaaa atggaagaat
401   attcatttat caaagtattc gctccatggt ctgatagatc ttttttttga
451   cgaactgctc aatcggacga gaataaagat agagtcccat tctacatgtc
501   aatactgaca acaatgaaat ttatagtatg aggaaaatcc gtcgacttta
551   gaaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details