JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729773.1
Database:	nt	Go to original record
Description:	Reaumuria cistoides tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria cistoides
Original size:	1014 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	572	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaatt attaaaatga gaagaaatga tggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgcgttgtta taagaattct
251   tccatcaaaa cttaaaaaaa gatcaagaca aaccctatat ttagataaat
301   acgtactgaa atactctaaa aaagaaaaaa acgtcttaat ggccaaaata
351   tgtcttttta tatgaaaaag tggaagaata ttcatttata aaagtattcg
401   ctccatggtc cgatagatct ttttttttga cgaactgctc aatcggacga
451   gaatgaagat agagtcccat tctacatgtc aatactgaca acaatgaaat
501   ttatagtatg aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details