JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729771.1
Database:	nt	Go to original record
Description:	Reaumuria botschantzevii clone 1 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria botschantzevii
Original size:	1035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	583	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaaaa ttattaaagt gagaagaaat gatggatagg tgcagagact
201   caacggaagc tgttctaaca aatggagttg actgcgttgt tataagaatt
251   cttccatcaa aacttaaaaa aagatcaaga caaaccctat atttagattt
301   agataaatac gtactgaaat actctaaaaa ataaaaaaac gtcttaatgg
351   gcggacaaaa tatgtctttt tatatgaaaa agtggaagaa tattcattta
401   taaaagtatt cactccatgg tccgatagat cttttttttg acgaactgct
451   caatcggacg agaatgaaga tagagtccca ttctacatgt caatactgac
501   aacaatgaaa tttatagtat gaggaaaatc cgtcgacttt agaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details