JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729770.1
Database:	nt	Go to original record
Description:	Reaumuria badhysi clone 1 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Reaumuria badhysi
Original size:	1030 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	589	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aaaaaccctg
101   gaattaataa aaatgggcaa tcctgagcca actcctgttt tcaaaacagt
151   aaaaaaaaat tattaaagtg agaagaaatg atggataggt gcagagactc
201   aacggaagct gttctaacaa atggagttga ctgcggttgt tataagaatt
251   cttcctcaaa acttaaaaaa agatcaagaa aaaccctata tttagattta
301   gataaatacg tactgaaata ctctaaaaaa gaaaaaaacg tcttaatgat
351   aatgacggcc aaaatatgtc ttttttatat gaaaaaatgg aagaatattc
401   atttatcaaa gtattcgctc catggtctga tagatctttt tttttgacga
451   actgctcaat cggacgagaa tgaagataga gtcccattct acatgtcaat
501   actgacaaca atgaaattta tagtatgagg aaaatccgtc gactttagaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details