JRC GMO-Amplicons | EURL GMFF

Record ID:	OL449699.1
Database:	nt	Go to original record
Description:	Atriplex portulacoides chloroplast, complete genome.
Taxon:	Atriplex portulacoides
Original size:	153163 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46897	47494	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaatta gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaggatagg
151   tgcagagact caaaggaagt tgttctaaca aatgggtttg ggttgactgt
201   gttgttctaa gaatccttcc gtcaaaactc caatctaaaa agggtgaaga
251   atatactatg tcaaatgatt aatgacaccc cgaatctata tctattttgt
301   attttttttt ttctaatttt ttagtattta ttatttctat atttaataca
351   gaagatgaaa ttctcgctaa ataaaaattg cgaatatgaa atgaaaatat
401   tataggaaaa aagggaagaa tttttgtaaa ttgatttcaa attgaaaaag
451   aatcaaatat tcattcatca aatcattcac tcccgagaat aaagatagag
501   tcccattcta catgtcaata ctgacaagaa tgaaatttat agtaagagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details