JRC GMO-Amplicons | EURL GMFF

Record ID:	MT156379.1
Database:	nt	Go to original record
Description:	Salvia grandifolia chloroplast, complete genome.
Taxon:	Salvia grandifolia
Original size:	151118 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46252	46814	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgccg gtagaggatt ccatggaaac tttagaaagg
251   atgaaggata aacacatcta ttgaatacta tattaaatga ttaatgttga
301   cccgaatctg tttttttaat atgaaaatga aaaaatggaa aaataggtgt
351   gaatttattt cacgttgaag aataaatcga atattcctca attcattcac
401   tccatagtcc gatagatctt ttaaagaact tattaatcgg acgagaataa
451   agatagagtc ccattctaca tgctacatgt caataccggc aacaatgaaa
501   tttatggtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details