JRC GMO-Amplicons | EURL GMFF

Record ID:	OL397049.1
Database:	nt	Go to original record
Description:	Kalidium foliatum chloroplast, complete genome.
Taxon:	Kalidium foliatum
Original size:	153773 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	72297	72911	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa ctcctttttt caaaagaaaa aaaaaggata
151   ggtgcagaga ctcaaaggaa gttgttctaa caaatgggtt tgggttgact
201   gtgttgttct aagaattctt ccgtcaaaac tccaatccaa aaagggtgaa
251   gaatatacta tatcaaatga ttaatgacac cccgaatcta ttttgtattt
301   ttttctaatt ttttgatatt tattatttct atatttaata tcgaagatga
351   aattctcgct aaataaaaat tgcgaatatg aaatgaaaat ctatattaat
401   gaaaatctat attatatgaa taggaaaaaa ggaagaattt ttgtgaatcg
451   atttaaagtt gaaaaagaat caaatattca ttcatcaaat cattcactcc
501   cgagaataaa gatagagtcc cattctacat gtcaatactg acaagaatga
551   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details