JRC GMO-Amplicons | EURL GMFF

Record ID:	OK054490.1
Database:	nt	Go to original record
Description:	Fagopyrum urophyllum chloroplast, complete genome.
Taxon:	Fagopyrum urophyllum
Original size:	159350 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47741	48302	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   cggataatcc tgagccaact cctgctttcc aaaagaaaga ataaatacag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaagc ggagttgact
201   gtattgcgtt gttataagaa ttcttccgtc aaaactcaaa aaaggatgaa
251   gaataaacct atatgcatac gtacgtaaat actaaaaaaa aaataataat
301   attgatgaca acccggatat ttattttatg acaaagaaga tatgaatcaa
351   tttcaaattg aagaaaaaat taaatattca tttattatta ttaaaagcta
401   ctcccatccc aagtctgatg gatcttttaa agaaccgatc catcggatga
451   gaatgaagat agagtcccat tctacgtgtc aatcctgaca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta taaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details