Match details

Record ID: OK637269.1
Database: nt Go to original record
Description: Digitaria sanguinalis chloroplast, complete genome.
Taxon: Digitaria sanguinalis
Original size: 138079 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 46906 47515 + 610 bp

Amplicon: 610 bp
1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggtaatcct gagccaaatc ccttttttga aaaaacaagc ggttttcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcaaaat
251   tagaaaaaga agggctttat acatctaata cgtatagata ctgacatagc
301   aaacgattaa tcacaaaacc catatcataa tataggtttt ttatttgatt
351   ttttaaaatg aaattaggaa tgattatgaa atagaaaatt ctgaattttt
401   ttagaattat tgtgaatcca ttccaatcga atattgagta atcaaatcct
451   tcaattcatt attttcgaga tctttaaaaa agtggattaa tcgaacgagg
501   ataaagagag agtcccattc tacatgtcaa tactgacagc aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.75e-08
primerB 100.0 20 bp 0 0 610 591 2.75e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.