JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ958825.1
Database:	nt	Go to original record
Description:	Clerodendrum cyrtophyllum chloroplast, complete genome.
Taxon:	Clerodendrum cyrtophyllum
Original size:	152052 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47116	47673	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttgctaag tgatgacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgctg gtagaggaat ttttccatgg aaacttcaga
251   aaggatgaag gataaatgga tctattgaat actatatcga atgattaatg
301   atggcccgaa tctgtatttt ttaatatgaa aaatggaaga attggtgtga
351   attgattcca cattgaagaa aaaatcgaat attaattcat caaattattc
401   actccacagt ccgatagatc ttttcaagat ctgattaatc ggacgagaat
451   aaagatagag tcccgttcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details