JRC GMO-Amplicons | EURL GMFF

Record ID:	MK281588.1
Database:	nt	Go to original record
Description:	Siraitia grosvenorii chloroplast, complete genome.
Taxon:	Siraitia grosvenorii
Original size:	158757 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50171	50773	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa caaaaagggg
151   gtaggtgcag agactcaatg gaagctgttc taacaaatgg agttgactac
201   gttgcgttag taaaggaatg aatccttcca tcgaaactcc agaaaagatg
251   aaagataaac gtattacgta ctgaaatact atttgattaa tgacaacccg
301   aatctctatt ttttatattt atattttttt atttatataa aaaatgatat
351   ataaaaatta acgaattgtt atgaaccgat tccaatatct ccaagttgaa
401   aaaagaatcg aatattcatt gatcaaatca tttactccat catacatagt
451   ctgatagatc ttttgaagaa ctgattaatc agatgcgaat agaataaaga
501   tagagtccca ttctacatgt caataccgac aaaaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
601   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details