JRC GMO-Amplicons | EURL GMFF

Record ID:	OK129338.1
Database:	nt	Go to original record
Description:	Cucurbita maxima chloroplast, complete genome.
Taxon:	Cucurbita maxima
Original size:	157205 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50245	50786	+	542 bp

Amplicon:

542 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aagggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatcct tccaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atattgatta atgacaactc gaatctctat
301   tttttatatt tatatataaa aaataatata taaaaatgaa cgaattgtta
351   taaatcgatg atcaaatcat ttactccatc atacagagtc tgatagatct
401   tttgaagaac tgattaatca gatgcgaata gaataaagat agagtcccat
451   tctacatgtc aataccgaca aaaatgaaat ttatagtaag aggaaaatcc
501   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	542	523	2.44e-08

Match details