JRC GMO-Amplicons | EURL GMFF

Record ID:	OK040957.1
Database:	nt	Go to original record
Description:	Fallopia convolvulus chloroplast, complete genome.
Taxon:	Fallopia convolvulus
Original size:	162636 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49588	50196	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   tgggcaatcc tgagccaact ccttctttcc aaaaggaaga caaaaggata
151   ggtgcagaga ctcaatggaa gttgttctaa caaatggagt tgacggtctt
201   gcgttgttat aagaattctt ccatcgaaac ttcaaaaagg atgaagaata
251   aacctagacg catacgtact taaattaaat ataaataata ctctatttat
301   ataaaattaa actaaaattt ttagaatatt caaattaaat gaaaaatatg
351   gatgacgacc cgaatcttta ttttatgact atgaaaaagt ggaagaattg
401   ttgtgaattg attccaagtt taagaaagaa tcgaatattc gttttttagt
451   aaatgaaact agtccccagt ccgatagatc tccgacccat cggatgagaa
501   tgaagataga gtcccattct acgtgtcaat cctgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details