JRC GMO-Amplicons | EURL GMFF

Record ID:	OK040956.1
Database:	nt	Go to original record
Description:	Fallopia dentatoalata chloroplast, complete genome.
Taxon:	Fallopia dentatoalata
Original size:	162625 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49623	50242	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   tgggcaatcc tgagccaact ccttctttcc aaaaggaaga caaaaggata
151   ggtgcagaga ctcaatggaa gttgttctaa caaatggagt tgacggtctt
201   gcgttgttat aagaattctt ccatcgaaac tccaaaaagg atgaagaata
251   aacctagacg catacgtact taacttaaat ataaataata ctctatttat
301   ataaaattaa actaaaattt tttaaaattt ttagaatatt aaaattaaat
351   aaaaaaatat ggatgacgac ccgaatcttt attttatgat tatgaaaaag
401   tggaagaatt gttgtgaatt gattccaagt ttaagaaaga atcgaatatt
451   cgttttttag taaatgaaac tattccccag tccgatagat ctccgaccca
501   tcggatgaga atgaagatag agtcccattc tacgtgtcaa tcctgacaac
551   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details