JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ618352.1
Database:	nt	Go to original record
Description:	Fallopia aubertii chloroplast, complete genome.
Taxon:	Fallopia aubertii
Original size:	162393 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49677	50295	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag acaaaaggat
151   aggtgcagag actcaatgga agttgttcta acaaatggag ttgacggtct
201   tgcgttgtta taagaattct tccatcgaaa ctccaaaaag gatgaagaat
251   aaacctagac gcatacgtac tttaatttta tataaataat actctattta
301   tatatttata taaaattaaa ctaaaatatt tagaatattc aaattaaatg
351   aaaaaatatg gatgacgacc cgaatcttta ttttatgact atgaaaaagt
401   ggaagaattg ttgtgaattg attccaagtt taagaaagaa tcgaatattc
451   gttttttagt aaattaaatt attccccagt ccgatagatc tccgacccat
501   cggatgagaa tgaagataga gtcccattct acgtgtcaat cctgacaaca
551   atgaaattta tagtaaaagg aaaatccgtc gactttaaaa atcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details