JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ618350.1
Database:	nt	Go to original record
Description:	Pteroxygonum denticulatum chloroplast, complete genome.
Taxon:	Pteroxygonum denticulatum
Original size:	162928 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50541	51134	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggcaat cctgagccaa ctcctgcttt ccaaaaggaa gaataaaaaa
151   aggataggtg cagagactca atggaagctg ttctaacaaa tgatggagtt
201   gactgtcttg cgttgttata agaattcttc catcgaaact ccaaaaagaa
251   tgaagaataa acctataagc atacgtactt aaatacaaat actatatcat
301   atataatatt caatttaaaa atattgatga ggacccgaat cttgatttta
351   tgactatgaa aaaatggaag aattgctgtg aatcgattcc aagttgaaga
401   aagaatcgaa tattcgttta gtaaattaaa gcatttactc ccctgtctga
451   tagatctttt gaaaaaccga cccatcggat gagaatgaag atagagtccc
501   attctacgtg tcaatcctga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details