JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ680513.1
Database:	nt	Go to original record
Description:	Wendlandia sp. XPW101 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Wendlandia sp. XPW101
Original size:	1747 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	816	1380	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cattagtaga taaatctttc cgtctaaaat tccgaaaggt
251   aaagtgaagg ataaacgtat atatacgtat tgaatactat atcaaatgat
301   taatgacaac tcgactgaat ctgtattttt tatatcaaaa cggaagaatt
351   ggtgtgaata gattccacat tgaagaaaga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg atagatcttt tcaagaattt attaatcaga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details