JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ680507.1
Database:	nt	Go to original record
Description:	Wendlandia tinctoria subsp. intermedia voucher XPW109 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Wendlandia tinctoria subsp. intermedia
Original size:	1759 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	817	1382	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggtaat cctgagccaa atcccgtttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cattagtaga taaatctttc cgtctaaaat tccgaaagga
251   taaagtgaag gatagacgta tatatacgta ttgaatacta tatcaaatga
301   ttaatgacaa ctcgactgaa tctgtatttt ttatatgaaa acggaagaat
351   tggtgtgaat agatttcaca ttgaagaaag aatcgaatat tcattgatca
401   aatgattcac tccatagtct gatagatctt ttcaagaatt tattaatcag
451   acgagaataa agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details