JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ680506.1
Database:	nt	Go to original record
Description:	Wendlandia subalpina voucher XPW293 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Wendlandia subalpina
Original size:	1771 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	828	1394	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cattagtaga taaatctttc cgtctaaaat tccgaaagga
251   taaagtgaag gataaacgta tatatacgta ttgaatacta taatcaaatg
301   attagtgaca actcgactga atctgtattt tttatatgaa aacgtaagaa
351   ttggtgtgaa tagattccac attgaagaaa gaatcgaata ttcattgatc
401   aaatgattca ctccatagtc tgatagatct tttcaagaat ttattaatca
451   gacgagaata aagatagagt cccgttctac atgtcaatgt cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details