JRC GMO-Amplicons | EURL GMFF

Record ID:	JX161551.1
Database:	nt	Go to original record
Description:	Eritrichium nanum voucher population 10 tRNA-Leu (trnL) gene, intron; plastid.
Taxon:	Eritrichium nanum
Original size:	548 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	547	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atccggtttt ccgaaaacaa aagttgaaaa
151   agaaaaagga taggtgcaga gactcaatgg aagctgttct aataaatgga
201   gttgactgct ttggtcgaag aatctttctc ataaagtgtg aaagtatata
251   cgtattgaat actttataaa atataataaa atgattaatg actaatatat
301   tctgttttta tgaaaaaaag aagagttggt gttaatcgat tccacataga
351   agaaagaatc gaatattcat tgatcaaatc attcactcca tagtctgata
401   gatcttttct tttgaagaat tgattaatcg gacgagaata aagatagagt
451   cccgttctac atgtcaatac cggcaacaat gaaatttata gtaagaggaa
501   aatccgtcga ctttaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details