JRC GMO-Amplicons | EURL GMFF

Record ID:	KF269074.1
Database:	nt	Go to original record
Description:	Prostanthera densa voucher Wilson 173 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (tRNA-Leu) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Prostanthera densa
Original size:	1525 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	671	1241	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaacaaa
101   aatgggtaat cctgagccaa atcccgtttt tctcaaaaca aaggtaaagg
151   ttcaaaaaac gaaaaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgccctgata gaggcatctt tgcatggaaa
251   cttccgaaag gatgaaggat aaatgtatct attgaatact atatcaaatg
301   attaatgatg gtccgaatct gtatttgtat tttttaatat gaaaaatgga
351   agaattggtg tgaattgatt ccacattgaa gaaaaaatcg aatattcagt
401   cagcaaatga ttcactccat agtccgatag atcttttaaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details