JRC GMO-Amplicons | EURL GMFF

Record ID:	KF269072.1
Database:	nt	Go to original record
Description:	Prostanthera densa voucher Henwood 41 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (tRNA-Leu) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Prostanthera densa
Original size:	1410 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	671	1239	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaacccccgg aattaacaaa
101   atgggtaatc ctgagccaaa tcccgttttc tcaaaacaaa ggtaaaggtt
151   caaaaaacga aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaatag gagttgactg ccctgataga ggcatcnnng catggaaact
251   tnagaaagga tgaaggatac atgtatctan nnnnnnnnnn nnnnnnnnnn
301   nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tntaatatga aaaatngaag
351   aattggnntn nnnngattnc acnnngaaga aaaaatnnna tattcagtca
401   gcaaannatt ctctncatag tncgatagat ctttnnnngn nnngattaat
451   cggacgagaa taaagataga gtcccnnnnt acatgtcaan ncnggcnncn
501   atgaaattta tagtaagagg aaaatccgtn gnntnnacaa ntnnngnggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details