JRC GMO-Amplicons | EURL GMFF

Record ID:	MW861760.1
Database:	nt	Go to original record
Description:	Marsdenia tenacissima chloroplast, complete genome.
Taxon:	Gongronemopsis tenacissima
Original size:	164482 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	54099	54639	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttcagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggtt ttccatcgaa aattcagaaa ggatgaaaga tatacatacg
251   tatcgaatac tatatcaaat gattaatgcc aaccccaatg agtctgtatt
301   ttttctataa aaacggaaga attggtgtga ttcgattctt tcttcaatgt
351   ggaatcgaat attcattgat caaaagattc actccatagt ctgtagatcc
401   tcttttcaag aactggatta atcggacgag aataaagata gagtcccgtt
451   ctacatgtca atgctggcaa caatgcaatt ttgagtaaga ggaaaatccg
501   tcgacttaaa aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details