JRC GMO-Amplicons | EURL GMFF

Record ID:	MW900176.1
Database:	nt	Go to original record
Description:	Schizonepeta tenuifolia chloroplast, complete genome.
Taxon:	Schizonepeta tenuifolia
Original size:	151260 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46521	47077	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgacaaaa agggataggt gcagagactc aatggaagct gttctaacga
201   atggagttga ctgcgccggt agaagaatct ttccatggaa attttcgaaa
251   ggatgaagga taaacgcatc tattgaatac tatatcaaat gattaatgtt
301   ggcccgaatc tgttttttta atatgaaaat gaaaaaatca gtgtgaattt
351   attttacgtt gaagaaaaaa gagaatattc atcaactcat tcactccata
401   gtccggtaga tcttttaaag aactcattaa tcggacgaga ataaagatag
451   agtcccattc tacatgctac atatcaatac cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details