JRC GMO-Amplicons | EURL GMFF

Record ID:	MW900174.1
Database:	nt	Go to original record
Description:	Lilium hansonii chloroplast, complete genome.
Taxon:	Lilium hansonii
Original size:	152668 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45088	45705	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttttaa ttttaatata aaactacaat aaaaaaagat aggtgcagag
201   actcaatgga agttgttcta acgaatgaag ttgattacgt tgcgctggta
251   gccggaattc ttctatcaaa attacagaga ggatgcccgc cctatatacg
301   tatatataca tactgaccat agtaaacgat tgattaatcg cggcccgaat
351   ccattataat atatatatat atatgaaaaa tttaagagtt attgtaaatc
401   tattccatat tccaatcaaa gtttaaggaa gaatcgaata ttcagtgatc
451   aaattattca ttccagagtc tgtatagatc tttttaaaaa ctgattattc
501   ggacgagaat aaagagagag tcccattcta cgtgtcaata ccgacaacaa
551   tgaaatttat agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details