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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MT891155.1
Database: nt Go to original record
Description: Cloning vector pSN.5_mTagBFP2, complete sequence.
Taxon: Cloning vector pSN.5_mTagBFP2
Original size: 9290 bp

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 832 1025 + 194 bp
Amplicon: 194 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
51    cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
101   gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
151   tgatatctcc actgacgtaa gggatgacgc acaatcccac tatc

View other 35 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.08e-08
primerB 100.0 20 bp 0 0 194 175 9.23e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.