JRC GMO-Amplicons | EURL GMFF

Record ID:	MW242810.1
Database:	nt	Go to original record
Description:	Coronanthera pulchra chloroplast, partial genome.
Taxon:	Coronanthera pulchra
Original size:	152734 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47454	48014	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaatcaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atagaaaatg attaataacg
301   gcccgaatct gtatctgtat ttttgtatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatcg aatattcatg aatcaaataa
401   tgaactccat agtccgatag atcttttaaa gaacttatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca ataccggcaa caatgaaaca
501   actagtaaga ggaaaatccg tcgactttca aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details