JRC GMO-Amplicons | EURL GMFF

Record ID:	MT740257.1
Database:	nt	Go to original record
Description:	Pycnostachys reticulata isolate BOP018543 chloroplast, complete genome.
Taxon:	Pycnostachys reticulata
Original size:	152480 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46984	47533	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaagg ataggtgcag agactcaatg gaagctgttc taacaaatgg
201   gagttgactg cgctggtaga ggaatctttc catggaaact ttagaaagga
251   tgaaggataa acgcatctat tgaatactat atcaaatggt taatgatggc
301   ccgaatcttt taatatgaaa aatggaaaaa ttggtgtgaa ttgattctac
351   gttgaagaaa aaatcgaata ttcatcaact cattcgctcc atagtccgat
401   agatctttga aagaactgat taatcggacg agaataaaga tagagtccca
451   ttctacatgc tacatgtcaa taccggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details