JRC GMO-Amplicons | EURL GMFF

Record ID:	MT731954.1
Database:	nt	Go to original record
Description:	Galeopsis bifida isolate BOP018180 chloroplast, complete genome.
Taxon:	Galeopsis bifida
Original size:	152587 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47048	47609	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattc taatgaaatt gagccttagt
51    gtgggaaacc tgctaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaacc gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactcc gttggtagag gactcctttc tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatgatggc cgaatccgta ttttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga agaaaaaatc gaatattcat tcatcaaatc
401   attcactcca tagtccgata gatcttttaa ataactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aatactggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details