JRC GMO-Amplicons | EURL GMFF

Record ID:	MT731953.1
Database:	nt	Go to original record
Description:	Caryopteris tangutica isolate BOP018181 chloroplast, complete genome.
Taxon:	Caryopteris tangutica
Original size:	151542 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46732	47293	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaccaa aggttcaaaa
151   aacgaaaaga aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgt gctggtagag gaatctttcc atggaaactt
251   cagaaaggat gaaagataaa tgtatctatt gaatactata tcaaatgatt
301   aatgatggcc cgaatctgta ttttttaata tgaaaaatgg aagaattggt
351   gtgaattgat tccacattga agaaaaaatc gaatattcat tcatcaaatc
401   attcactcca tagtctgatc gatcttttca agaactgatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aataacggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details