JRC GMO-Amplicons | EURL GMFF

Record ID:	OK336484.1
Database:	nt	Go to original record
Description:	Cucurbita ficifolia chloroplast, complete genome.
Taxon:	Cucurbita ficifolia
Original size:	157631 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50322	50903	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aagggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga caacgttgcg
201   ttaataaagg aatgaatcct tccaaactcc agaaaagcag aaacataaac
251   gtattacgta ctgaaatact atattgatta atgacaactc gaatctctat
301   tttttatatt tatatataaa aaataatata taaaaatgaa cgaattgtta
351   taaatcgatt ccaatatctc caagttgaaa aaagaatcga atattcattg
401   atcaaatcat ttactccatc atacagagtc tgatagatct tttgaagaac
451   tgattaatca gatgcgaata gaataaagat agagtcccat tctacatgtc
501   aataccgaca aaaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   aaaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details