JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ328080.1
Database:	nt	Go to original record
Description:	Saccharum officinarum cultivar GT35 chloroplast, complete genome.
Taxon:	Saccharum officinarum
Original size:	141181 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49533	50058	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cccttttttg aaaaaacaag tggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaagtaataa cgattaatca cagaacccat attataatat
251   aggttcttta ttttattttt agaatgaaat taggaatgat tatgaaatag
301   aaaattcata atttttttag aattattgtg aatctattcc aatcgaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatctt ttaaaaagtg
401   gattaatcgg acgaggataa agagagagtc ccattctaca tgtcaatact
451   gacaacaatg aaatttctag taaaaggaaa atccgtcgac tttataagtc
501   gtgagggttc aagtccctct atcccc

View other 141 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details