JRC GMO-Amplicons | EURL GMFF

Record ID:	MT727082.1
Database:	nt	Go to original record
Description:	Momordica charantia chloroplast, complete genome.
Taxon:	Momordica charantia
Original size:	161949 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50376	50978	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaag gggggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatgga atgaatcctt ccatcgaaac tccagaaaag
251   atgaaagata aacgtattac gtactgaaat actatttgat taatgacgac
301   ccgaatctct attttttata tttatatttt tatataaaat ataaaaaaat
351   ataaaaatga acgaattgtt atgaatcgat tccaatatct ccaagttgaa
401   aaaagaatcg aatattcatt gatcaaatca tttactccat catacatagt
451   ctgatagatc ttttgaagaa ctgattaatc agatgcgaat agaataaaga
501   tagagtccca ttctacatgt caataccgac aaaaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details