JRC GMO-Amplicons | EURL GMFF

Record ID:	MN810989.1
Database:	nt	Go to original record
Description:	Fritillaria walujewii chloroplast, complete genome.
Taxon:	Fritillaria walujewii
Original size:	151923 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44741	45347	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa gataggtgca gagactcaat
201   ggaagctgtt ctaacgaatg aagttgatta cgttgcgctg gtagccggaa
251   tccttctatc aaaattacgg agaggatgcc cgccctatat acgtatacat
301   acatactgac atagtaaacg attgattaat cgcggcccga atccattata
351   atatatatat atgaaaaatt taagagttat tgtaaatcta ttccatattc
401   caatcaaagt ttaaggaaga atcgaaatat tcagtgatca aattattcat
451   tccagagtct gtatagatct ttttaaaaac tgattattcg gacgagaata
501   aagagagagt cccattctac gtgtcaatac cgacaacaat gaaatttata
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details