JRC GMO-Amplicons | EURL GMFF

Record ID:	MN810987.1
Database:	nt	Go to original record
Description:	Fritillaria tortifolia chloroplast, complete genome.
Taxon:	Fritillaria tortifolia
Original size:	151943 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44825	45432	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccgccctata tacgtatata
301   tacatactga catagtaaac gattgattaa tcgcggcccg aatctattat
351   aatatatata tatgaaaaat ttaagagtta ttgtaaatct attccatatt
401   ccaatcaaag tttaaggaag aatcgaaata ttcagtgatc aaattattca
451   ttccagagtc tgtatagatc tttttaaaaa ctgattattc ggacgagaat
501   aaagagagag tcccattcta cgtgtcaata ccgacaacaa tgaaatttat
551   agtaaaagga aaatccgtcg actttataag tcgtgagggt tcaagtccct
601   ctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details